Bakkes PJ, Lenz P, Müller C, Bida A, Dohmen-Olma D, Knapp A, Oldiges M, Jaeger KE, Freudl R (2021) Biosensor-based optimization of cutinase secretion by Corynebacterium glutamicum. Front Bioeng Biotechnol 9: 750150. https://doi.org/10.3389/fmicb.2021.750150

Bakkes PJ, Ramp P, Bida A, Dohmen-Olma, Bott M, Freudl R (2020) Improved pEKEx2-derived expression vectors for tightly controlled production of recombinant proteins in Corynebacterium glutamicum. Plasmid 112: 102540. https://doi.org/10.1016/j.plasmid.2020.102540

Baumer B, Classen T, Pohl M, Pietruszka J (2020) Efficient Nicotinamide Adenine Dinucleotide Phosphate [NADP(H)] Recycling in Closed-Loop Continuous Flow Biocatalysis. Adv Synth Catal 362: 2894-2901 https://doi.org/10.1002/adsc.202000058

Becker M, Lütz S, Rosenthal K (2021) Environmental Assessment of Enzyme Production and Purification. Molecules 26: 573. https://doi.org/10.3390/molecules26030573

Becker M, Nikel P, Andexer JN, Lütz S, Rosenthal K (2021) A Multi-Enzyme Cascade Reaction for the Production of 2’3’-cGAMP. Biomolecules 11(4): 590. https://doi.org/10.3390/biom11040590

Bednarz H, Roloff N, Niehaus K (2019) Mass spectrometry imaging of the spatial and temporal localization of alkaloids in nightshades. J Agric Food Chem 67: 13470-13477. https://doi.org/10.1021/acs.jafc.9b01155

Burgardt A, Moustafa A, Persicke M, Sproß J, Patschkowski T, Risse JM, Peters-Wendisch P, Lee J-H, Wendisch VF (2021) Coenzyme Q10 biosynthesis established in the non-ubiquinone containing Corynebacterium glutamicum by metabolic engineering. Front Biotechnol Bioeng 9: 650961 https://doi.org/10.3389/fbioe.2021.650961

Burgardt A, Pelosi L, Chehade MH, Wendisch VF, Pierrel F (2022) Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production. Metabolites 12: 428. https://doi.org/10.3390/metabo12050428

Burgardt A, Prell C, Wendisch VF (2021) Utilization of a wheat sidestream for 5-aminovalerate production by Corynebacterium glutamicum. Front Bioeng Biotechnol 9: 732271. https://doi.org/10.3389/fbioe.2021.732271

David B, Schneider P, Schäfer P, Pietruszka J, Gohlke H (2021) Discovery of new acetylcholinesterase inhibitors for Alzheimer’s disease: virtual screening and in vitro characterisation. J Enz Inhib Med Chem 36: 491-496; https://doi.org/10.1080/14756366.2021.1876685

Dementyeva P, Freudenberg RA, Baier T, Rojek K, Wobbe L, Weisshaar B, Kruse O (2021) A novel, robust and mating-competent Chlamydomonas reinhardtii strain with an enhanced transgene expression capacity for algal biotechnology. Biotechnol Rep 31:e00644. https://doi.org/10.1016/j.btre.2021.e00644

Dietsch M, Behle A, Westhoff P, Axmann IM (2021) Metabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction of the sesquiterpene valencene. Metab Eng Commun 13:e00178. https://doi.org/10.1016/j.mec.2021.e00178

El Harrar T, Frieg B, Davari MD, Jaeger KE, Schwaneberg U, Gohlke H (2021) Aqueous ionic liquids redistribute local enzyme stability via long-range perturbation pathways. Comp Struct Biotechnol J 19: 4248–4264. https://doi.org/10.1016/j.csbj.2021.07.001

Fejzagić AV, Gebauer J, Huwa N, Classen T (2019) Halogenating Enzymes for Active Agent Synthesis: First Steps Are Done and Many Have to Follow. Molecules 24: 4008; https://doi.org/10.3390/molecules24214008

Fejzagić AV, Myllek S, Hogenkamp F, Greb J, Pietruszka J, Classen T (2020) A fluorescence‐based assay system for the determination of haloperoxidase‐activity using a two‐dimensional calibration approach. Chem Open 9: 959-966; https://doi.org/10.1002/open.202000184

Freudenberg RA, Baier T, Einhaus A, Wobbe L, Kruse O (2021) High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii. Biores Technol 323: 124542  https://doi.org/10.1016/j.biortech.2020.124542

Freudenberg RA, Wittemeier L, Einhaus A, Baier T, Kruse O. The Spermidine Synthase Gene SPD1: A Novel Auxotrophic Marker for Chlamydomonas reinhardtii Designed by Enhanced CRISPR/Cas9 Gene Editing. Cells. 2022; 11(5):837. https://doi.org/10.3390/cells11050837

Göttl V, Schmitt I, Braun K, Peters-Wendisch P, Wendisch VF, Henke NA (2021) CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum. Microorganisms, 9: 670 https://doi.org/10.3390/microorganisms9040670.

Henke NA, Göttl V, Schmitt I, Peters-Wendisch P, Wendisch VF (accepted) A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi
In: Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology. Wurtzel E (Ed); Methods in Enzymology, 670. Elsevier : 412. https://doi.org/10.1016/bs.mie.2021.11.26

Hilgers F, Habash SS, Loeschcke A, Ackermann YS, Neumann S, Heck A, Klaus O, Hage-Hülsmann J, Grundler FMW, Jaeger KE, Schleker ASS, Drepper T (2021) Heterologous Production of β-Caryophyllene and Evaluation of Its Activity against Plant Pathogenic Fungi. Microorganisms 9: 168. https://doi.org/10.3390/microorganisms9010168

Hogenkamp F, Hilgers F, Knapp A, Klaus O, Bier C, Binder D, Jaeger KE, Drepper T, Pietruszka J (2020) Effect of photocaged isopropyl β‐D‐1‐thiogalactopyranoside solubility on the light responsiveness of LacI‐controlled expression systems in different bacteria. ChemBioChem 22: 539-547; https://doi.org/10.1002/cbic.202000377

Hussnaetter KP, Philipp M, Müntjes K, Feldbrügge M, Schipper K (2021) Controlling unconventional secretion for production of heterologous proteins in Ustilago maydis through transcriptional regulation and chemical inhibition of the kinase Don3. J Fungi 7:179. https://doi.org/10.3390/jof7030179

Jäger VD, Lamm R, Küsters K, Ölcücü G, Oldiges M, Jaeger KE, Büchs J, Krauss U (2020) A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum. Appl Microb Biotechnol 104: 7313-7329; https://doi.org/10.1007/s00253-020-10760-3

Jurischka S, Bida A, Dohmen-Olma D, Kleine B, Potzkei J, Binder S, Schaumann G, Bakkes PJ, Freudl, R (2020) A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum. Microb Cell Fact 19: 11; https://doi.org/10.1186/s12934-019-1273-z

Keysberg C, Hertel O, Schelletter L, Busche T, Sochart C, Kalinowski J, Hoffrogge R, Otte K, Noll T (2021) Exploring the molecular content of CHO exosomes during bioprocessing. Appl Microbiol Biotechnol 105: 3673-3689. https://doi.org/10.1007/s00253-021-11309-8

Kinner A, Nerke P, Siedentop R, Steinmetz T, Classen T, Rosenthal K, Nett M, Pietruszka J, Lütz S (2022) Recent Advances in Biocatalysis for Drug Synthesis. Biomedicines 10: 964, https://doi.org/10.3390/biomedicines10050964

Klein AS, Classen T, Pietruszka J (2020) On Biocatalysis as Resourceful Methodology for Complex Syntheses: Selective Catalysis, Cascades and Biosynthesis. In: Pharmaceutical Biocatalysis: Fundamentals, Enzyme Inhibitors, and Enzymes in Health and Diseases. Grunwald P (Ed); CRC Press, Boca Raton, FL, USA. https://doi.org/10.1201/9780429295034-21

Kokorin A, Parshin PD, Bakkes PJ, Pometun AA, Tishkov VI, Urlacher VB (2021) Genetic fusion of P450 BM3 and formate dehydrogenase towards self-sufficient biocatalysts with enhanced activity. Sci Rep 11:21706, https://doi.org/10.1038/s41598-021-00957-5

Küsters K, Pohl M, Krauss U, Ülcücü G, Albert S, Wiechert W, Oldiges M (2021) Construction and comprehensive characterization of an EcLDCc-CatIB set—varying linkers and aggregation inducing tags. Microb Cell Fact 20: 49. https://doi.org/10.1186/s12934-021-01539-w

Luttermann T, Rückert C, Wibberg D, Busche T, Schwarzhans JP, Friehs K, Kalinowski J (2021) Establishment of a near-contiguous genome sequence of the citric acid producing yeast Yarrowia lipolytica DSM 3286 with resolution of rDNA clusters and telomeres.  NAR Genom Bioinform 3: lqab085. https://doi.org/10.1093/nargab/lqab085

Mindt M, Walter T, Kugler P, Wendisch VF (2020) Microbial engineering for production of N-functionalized amino acids and amines. Biotechnol J 15: 1900451. https://doi.org/10.1002/biot.201900451

Müller C, Igwe CL, Wiechert W, Oldiges M (2021) Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay, Microbial Cell Factories, 20: 191 https://doi.org/10.1186/s12934-021-01672-6

Nutschel C, Coscolín C, Mulnaes D, David B, Ferrer M, Jaeger KE, Gohlke H (2021) Promiscuous esterases counterintuitively are less flexible than specific ones. J Chem Inf Model 65: 22383-2395. https://doi.org/10.1021/acs.jcim.1c00152

Ölçücü G, Klaus O, Jaeger KE, Drepper T, Krauss U (2021) Emerging solutions for in vivo biocatalyst immobilization: tailor-made catalysts for industrial biocatalysis. ACS Sustainable Chem Eng 9: 8919–8945. https://doi.org/10.1021/acssuschemeng.1c02045

Pierrel F, Burgardt A, Lee J-H, Pelosi L, Wendisch VF (2022) Recent Advances in the Metabolic Pathways and Microbial Production of Coenzyme Q. World J Microbiol Biotechnol 38: 58. https://doi.org/10.1007/s11274-022-03242-3

Prell C, Burgardt A, Meyer F, Wendisch VF (2021) Fermentative production of ʟ-2-hydroxyglutarate by engineered Corynebacterium glutamicum via pathway extension of ʟ-lysine biosynthesis. Front Bioeng Biotechnol 8: 630476. https://doi.org/10.3389/fbioe.2020.630476

Prell C, Busche T, Rückert C, Nolte L, Brandenbusch C, Wendisch VF (2021) Adaptive Laboratory Evolution accelerated glutarate production by Corynebacterium glutamicum. Microb Cell Fact 20: 97. https://doi.org/10.1186/s12934-021-01586-3

Prell C, Vonderbank S, Meyer F, Perez F, Wendisch VF (2022) Metabolic engineering of Corynebacterium glutamicum for de novo production of 3-hydroxycadaverine. Curr Res Biotechnol 4: 32-46. https://doi.org/10.1016/j.crbiot.2021.12.004

Ramp P, Lehnert A, Matamouros S, Wirtz A, Baumgart M, Bott M (2021) Metabolic engineering of Corynebacterium glutamicum for production of scyllo-inositol, a drug candidate against Alzheimer's disease. Metab Eng  67:173-185. https://doi.org/10.1016/j.ymben.2021.06.011

Rolf J, Nerke P, Britner A, Krick S, Lütz S, Rosenthal K (2021) From Cell-Free Protein Synthesis to Whole-Cell Biotransformation: Screening and Identification of Novel α-Ketoglutarate-Dependent Dioxygenases for Preparative-Scale Synthesis of Hydroxy-L-Lysine. Catalysts 11:1038. https://doi.org/10.3390/catal11091038

Rosenthal K, Becker M, Rolf J, Siedentop R, Hillen M, Nett M, Lütz S (2020) Catalytic promiscuity of cGAS: A facile enzymatic synthesis of 2’‐3’ linked cyclic dinucleotides. ChemBioChem 21: 3225-3228. https://doi.org/10.1002/cbic.202000433

Schmitz J, Hertel O, Yermakov B, Noll T, Grünberger A (2021) Growth and eGFP-production of CHO-K1 suspension cells cultivated from single-cell to lab-scale. Front Bioeng Biotechnol 9: 716343. https://doi.org/10.3389/fbioe.2021.716343

Schmitz LM, Hageneier F, Rosenthal K, Busche T, Brandt D, Kalinowski J, Lütz S (2021) Recombinant Expression and Characterization of Novel P450s from Actinosynnema mirum. Bioorganic & Medicinal Chemistry 42:116241. https://doi.org/10.1016/j.bmc.2021.116241

Schmitz LM, Kinner A, Althoff K, Rosenthal K, Lütz S (2021) Investigation of vitamin D2 and vitamin D3 hydroxylation by Kutzneria albida. ChemBioChem 22: 2266-2274. https://doi.org/10.1002/cbic.202100027

Schmitz LM, Schäper J, Rosenthal K, Lütz S (2019) Accessing the biocatalytic potential for C-H-activation by targeted genome mining and screening, ChemCatChem 11: 5766-5777. https://doi.org/10.1002/cctc.201901273

Schneider P, Henßen B, Paschold B, Chapple BP, Schatton M, Seebeck F, Classen T, Pietruszka J (2021) Biocatalytic C3-Indole Methylation - A Useful Tool for the Natural Product Inspired Stereoselective Synthesis of Pyrroloindoles. Angew Chem Int Ed  https://doi.org/10.1002/anie.202107619

Schultenkämper K, Fernandes de Brito L, Wendisch VF (2020) Impact of CRISPR interference on strain development in biotechnology. Biotechnol Appl Biochem 67: 7-21. https://doi.org/10.1002/bab.1901

Seide S, Arnold L, Wetzels S, Bregu M, Gätgens J, Pohl M (2022) From Enzyme to Preparative Cascade Reactions with Immobilized Enzymes: Tuning Fe(II)/α-Ketoglutarate-Dependent Lysine Hydroxylases for Application in Biotransformations. Catal 12:354, https://doi.org/10.3390/catal12040354

Sester A, Winand L, Pace S, Hiller W, Werz O, Nett M (2019) Myxochelin- and Pseudochelin-Derived Lipoxygenase Inhibitors from a Genetically Engineered Myxococcus xanthus Strain, J Nat Prod 82: 2544-2549. https://doi.org/10.1021/acs.jnatprod.9b00403

Sester A, Stüer-Patowsky K, Hiller W, Kloss F, Lütz S, Nett M (2020) Biosynthetic plasticity enables production of fluorinated aurachins. ChemBioChem 21: 2268-2273; https://doi.org/10.1002/cbic.202000166

Sgobba E, Wendisch VF (2020) Synthetic microbial consortia for small molecule production. Curr Opin Biotechnol 62: 72–79. https://doi.org/10.1016/j.copbio.2019.09.011

Steenweg C, Seifert AI, Schembecker G, Wohlgemuth K (2021) Characterization of a Modular Continuous Vacuum Screw Filter for Small-Scale Solid–Liquid Separation of Suspensions. Org Process Res Dev 25, 926-940. https://doi.org/10.1021/acs.oprd.0c00550

Volkenborn K, Kuschmierz L, Benz N, Lenz P, Knapp A, Jaeger KE (2020) The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis. Microb Cell Fact 19: 154. https://doi.org/10.1186/s12934-020-01404-2

Wendisch VF (2020) Genome-reduced Corynebacterium glutamicum fit for biotechnological applications. In: Minimal Cells: Design, Construction, Biotechnological Applications. Lara AR, Gosset Lagarda G (Eds); Cham: Springer: 95-116. https://doi.org/10.1007/978-3-030-31897-0_4

Wendisch VF (2020) Metabolic engineering advances and prospects for amino acid production. Metab Eng. 58: 17-34. https://doi.org/10.1016/j.ymben.2019.03.008

Wendisch VF, Lee J-H (2020) Metabolic engineering in Corynebacterium glutamicum. In: Corynebacterium glutamicum – Biology and Biotechnology. Inui M, Toyoda K (Eds); Microbiology Monographs Vol 23. Springer, Cham: 287-322 https://doi.org/10.1007/978-3-030-39267-3_10

Wendisch VF, Nampoothiri KM, Lee J-H (2022) Metabolic engineering for valorization of agri-and aqua-culture sidestreams for production of nitrogenous compounds by Corynebacterium glutamicum. Front. Microbiol. in press. https://doi.org/10.3389/fmicb.2022.835131

Winand L, Schneider P, Kruth S, Greven N-J, Hiller W, Kaiser M, Pietruszka J, Nett M (2021) Mutasynthesis of physostigmines in Myxococcus xanthus. Org Lett 23, 6563-6567. https://doi.org/10.1021/acs.orglett.1c02374

Winand L, Sester A, Nett M (2021) Bioengineering of anti-inflammatory natural products. Chem Med Chem 16, 767-776. https://doi.org/10.1002/cmdc.202000771

Wierckx N, Miebach K, Ihling N, Hussnaetter KP, Büchs J, Schipper K. (2021) Perspectives for the application of Ustilaginaceae as biotech cell factories. Essays Biochem 65:365-379. https://doi.org/10.1042/EBC20200141

Witte KE, Hertel O, Windmöller BA, Helweg LP, Höving AL, Knabbe C, Busche T, Greiner JFW, Kalinowski J, Noll T, Mertzlufft F, Beshay M, Pfitzenmaier J, Kaltschmidt B, Kaltschmidt C, Banz-Jansen C, Simon M (2021) Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations. Cancers 13, 1136. https://doi.org/10.3390/cancers13051136

Wolf S, Becker J, Tsuge Y, Kawaguchi H, Kondo A, Marienhagen J, Bott M, Wendisch VF, Wittmann C (2021) Advances in metabolic engineering of Corynebacterium glutamicum to produce high-value active ingredients for food, feed, human health, and well-being. Essays Biochem 65: 197-212. https://doi.org/10.1042/EBC20200134