Bakkes PJ, Lenz P, Müller C, Bida A, Dohmen-Olma D, Knapp A, Oldiges M, Jaeger KE, Freudl R (2021) Biosensor-based optimization of cutinase secretion by Corynebacterium glutamicum. Front Bioeng Biotechnol 9: 750150. https://doi.org/10.3389/fmicb.2021.750150

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Bakkes PJ, Ramp P, Bida A, Dohmen-Olma, Bott M, Freudl R (2020) Improved pEKEx2-derived expression vectors for tightly controlled production of recombinant proteins in Corynebacterium glutamicum. Plasmid 112: 102540. https://doi.org/10.1016/j.plasmid.2020.102540

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Baumer B, Classen T, Pohl M, Pietruszka J (2020) Efficient Nicotinamide Adenine Dinucleotide Phosphate [NADP(H)] Recycling in Closed-Loop Continuous Flow Biocatalysis. Adv Synth Catal 362: 2894-2901 https://doi.org/10.1002/adsc.202000058

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Becker M, Lütz S, Rosenthal K (2021) Environmental Assessment of Enzyme Production and Purification. Molecules 26: 573. https://doi.org/10.3390/molecules26030573

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Becker M, Nikel P, Andexer JN, Lütz S, Rosenthal K (2021) A Multi-Enzyme Cascade Reaction for the Production of 2’3’-cGAMP. Biomolecules 11(4): 590. https://doi.org/10.3390/biom11040590

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Bednarz H, Roloff N, Niehaus K (2019) Mass spectrometry imaging of the spatial and temporal localization of alkaloids in nightshades. J Agric Food Chem 67: 13470-13477. https://doi.org/10.1021/acs.jafc.9b01155

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Burgardt A, Moustafa A, Persicke M, Sproß J, Patschkowski T, Risse JM, Peters-Wendisch P, Lee J-H, Wendisch VF (2021) Coenzyme Q10 biosynthesis established in the non-ubiquinone containing Corynebacterium glutamicum by metabolic engineering. Front Biotechnol Bioeng 9: 650961 https://doi.org/10.3389/fbioe.2021.650961

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Burgardt A, Pelosi L, Chehade MH, Wendisch VF, Pierrel F (2022) Rational Engineering of Non-Ubiquinone Containing Corynebacterium glutamicum for Enhanced Coenzyme Q10 Production. Metabolites 12: 428. https://doi.org/10.3390/metabo12050428

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Burgardt A, Prell C, Wendisch VF (2021) Utilization of a wheat sidestream for 5-aminovalerate production by Corynebacterium glutamicum. Front Bioeng Biotechnol 9: 732271. https://doi.org/10.3389/fbioe.2021.732271

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David B, Schneider P, Schäfer P, Pietruszka J, Gohlke H (2021) Discovery of new acetylcholinesterase inhibitors for Alzheimer’s disease: virtual screening and in vitro characterisation. J Enz Inhib Med Chem 36: 491-496; https://doi.org/10.1080/14756366.2021.1876685

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Dementyeva P, Freudenberg RA, Baier T, Rojek K, Wobbe L, Weisshaar B, Kruse O (2021) A novel, robust and mating-competent Chlamydomonas reinhardtii strain with an enhanced transgene expression capacity for algal biotechnology. Biotechnol Rep 31:e00644. https://doi.org/10.1016/j.btre.2021.e00644

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Dietsch M, Behle A, Westhoff P, Axmann IM (2021) Metabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction of the sesquiterpene valencene. Metab Eng Commun 13:e00178. https://doi.org/10.1016/j.mec.2021.e00178

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El Harrar T, Frieg B, Davari MD, Jaeger KE, Schwaneberg U, Gohlke H (2021) Aqueous ionic liquids redistribute local enzyme stability via long-range perturbation pathways. Comp Struct Biotechnol J 19: 4248–4264. https://doi.org/10.1016/j.csbj.2021.07.001

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Fejzagić AV, Gebauer J, Huwa N, Classen T (2019) Halogenating Enzymes for Active Agent Synthesis: First Steps Are Done and Many Have to Follow. Molecules 24: 4008; https://doi.org/10.3390/molecules24214008

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Fejzagić AV, Myllek S, Hogenkamp F, Greb J, Pietruszka J, Classen T (2020) A fluorescence‐based assay system for the determination of haloperoxidase‐activity using a two‐dimensional calibration approach. Chem Open 9: 959-966; https://doi.org/10.1002/open.202000184

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Freudenberg RA, Baier T, Einhaus A, Wobbe L, Kruse O (2021) High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii. Biores Technol 323: 124542  https://doi.org/10.1016/j.biortech.2020.124542

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Freudenberg RA, Wittemeier L, Einhaus A, Baier T, Kruse O. The Spermidine Synthase Gene SPD1: A Novel Auxotrophic Marker for Chlamydomonas reinhardtii Designed by Enhanced CRISPR/Cas9 Gene Editing. Cells. 2022; 11(5):837. https://doi.org/10.3390/cells11050837

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Göttl V, Schmitt I, Braun K, Peters-Wendisch P, Wendisch VF, Henke NA (2021) CRISPRi-library guided target identification for engineering carotenoid production by Corynebacterium glutamicum. Microorganisms, 9: 670 https://doi.org/10.3390/microorganisms9040670.

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Henke NA, Göttl V, Schmitt I, Peters-Wendisch P, Wendisch VF (accepted) A synthetic biology approach to study carotenoid production in Corynebacterium glutamicum: read-out by a genetically encoded biosensor combined with perturbing native gene expression by CRISPRi
In: Carotenoids: Carotenoid and apocarotenoid biosynthesis, metabolic engineering and synthetic biology. Wurtzel E (Ed); Methods in Enzymology, 670. Elsevier : 412. https://doi.org/10.1016/bs.mie.2021.11.26

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Hilgers F, Habash SS, Loeschcke A, Ackermann YS, Neumann S, Heck A, Klaus O, Hage-Hülsmann J, Grundler FMW, Jaeger KE, Schleker ASS, Drepper T (2021) Heterologous Production of β-Caryophyllene and Evaluation of Its Activity against Plant Pathogenic Fungi. Microorganisms 9: 168. https://doi.org/10.3390/microorganisms9010168

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Hogenkamp F, Hilgers F, Knapp A, Klaus O, Bier C, Binder D, Jaeger KE, Drepper T, Pietruszka J (2020) Effect of photocaged isopropyl β‐D‐1‐thiogalactopyranoside solubility on the light responsiveness of LacI‐controlled expression systems in different bacteria. ChemBioChem 22: 539-547; https://doi.org/10.1002/cbic.202000377

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Hussnaetter KP, Philipp M, Müntjes K, Feldbrügge M, Schipper K (2021) Controlling unconventional secretion for production of heterologous proteins in Ustilago maydis through transcriptional regulation and chemical inhibition of the kinase Don3. J Fungi 7:179. https://doi.org/10.3390/jof7030179

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Jäger VD, Lamm R, Küsters K, Ölcücü G, Oldiges M, Jaeger KE, Büchs J, Krauss U (2020) A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum. Appl Microb Biotechnol 104: 7313-7329; https://doi.org/10.1007/s00253-020-10760-3

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Jurischka S, Bida A, Dohmen-Olma D, Kleine B, Potzkei J, Binder S, Schaumann G, Bakkes PJ, Freudl, R (2020) A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum. Microb Cell Fact 19: 11; https://doi.org/10.1186/s12934-019-1273-z

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Keysberg C, Hertel O, Schelletter L, Busche T, Sochart C, Kalinowski J, Hoffrogge R, Otte K, Noll T (2021) Exploring the molecular content of CHO exosomes during bioprocessing. Appl Microbiol Biotechnol 105: 3673-3689. https://doi.org/10.1007/s00253-021-11309-8

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Kinner A, Nerke P, Siedentop R, Steinmetz T, Classen T, Rosenthal K, Nett M, Pietruszka J, Lütz S (2022) Recent Advances in Biocatalysis for Drug Synthesis. Biomedicines 10: 964, https://doi.org/10.3390/biomedicines10050964

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Klein AS, Classen T, Pietruszka J (2020) On Biocatalysis as Resourceful Methodology for Complex Syntheses: Selective Catalysis, Cascades and Biosynthesis. In: Pharmaceutical Biocatalysis: Fundamentals, Enzyme Inhibitors, and Enzymes in Health and Diseases. Grunwald P (Ed); CRC Press, Boca Raton, FL, USA. https://doi.org/10.1201/9780429295034-21

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Kokorin A, Parshin PD, Bakkes PJ, Pometun AA, Tishkov VI, Urlacher VB (2021) Genetic fusion of P450 BM3 and formate dehydrogenase towards self-sufficient biocatalysts with enhanced activity. Sci Rep 11:21706, https://doi.org/10.1038/s41598-021-00957-5

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Küsters K, Pohl M, Krauss U, Ülcücü G, Albert S, Wiechert W, Oldiges M (2021) Construction and comprehensive characterization of an EcLDCc-CatIB set—varying linkers and aggregation inducing tags. Microb Cell Fact 20: 49. https://doi.org/10.1186/s12934-021-01539-w

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Luttermann T, Rückert C, Wibberg D, Busche T, Schwarzhans JP, Friehs K, Kalinowski J (2021) Establishment of a near-contiguous genome sequence of the citric acid producing yeast Yarrowia lipolytica DSM 3286 with resolution of rDNA clusters and telomeres.  NAR Genom Bioinform 3: lqab085. https://doi.org/10.1093/nargab/lqab085

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Mindt M, Walter T, Kugler P, Wendisch VF (2020) Microbial engineering for production of N-functionalized amino acids and amines. Biotechnol J 15: 1900451. https://doi.org/10.1002/biot.201900451

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Müller C, Igwe CL, Wiechert W, Oldiges M (2021) Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay, Microbial Cell Factories, 20: 191 https://doi.org/10.1186/s12934-021-01672-6

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Nutschel C, Coscolín C, Mulnaes D, David B, Ferrer M, Jaeger KE, Gohlke H (2021) Promiscuous esterases counterintuitively are less flexible than specific ones. J Chem Inf Model 65: 22383-2395. https://doi.org/10.1021/acs.jcim.1c00152

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Ölçücü G, Klaus O, Jaeger KE, Drepper T, Krauss U (2021) Emerging solutions for in vivo biocatalyst immobilization: tailor-made catalysts for industrial biocatalysis. ACS Sustainable Chem Eng 9: 8919–8945. https://doi.org/10.1021/acssuschemeng.1c02045

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Pierrel F, Burgardt A, Lee J-H, Pelosi L, Wendisch VF (2022) Recent Advances in the Metabolic Pathways and Microbial Production of Coenzyme Q. World J Microbiol Biotechnol 38: 58. https://doi.org/10.1007/s11274-022-03242-3

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Prell C, Burgardt A, Meyer F, Wendisch VF (2021) Fermentative production of ʟ-2-hydroxyglutarate by engineered Corynebacterium glutamicum via pathway extension of ʟ-lysine biosynthesis. Front Bioeng Biotechnol 8: 630476. https://doi.org/10.3389/fbioe.2020.630476

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Prell C, Busche T, Rückert C, Nolte L, Brandenbusch C, Wendisch VF (2021) Adaptive Laboratory Evolution accelerated glutarate production by Corynebacterium glutamicum. Microb Cell Fact 20: 97. https://doi.org/10.1186/s12934-021-01586-3

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Prell C, Vonderbank S, Meyer F, Perez F, Wendisch VF (2022) Metabolic engineering of Corynebacterium glutamicum for de novo production of 3-hydroxycadaverine. Curr Res Biotechnol 4: 32-46. https://doi.org/10.1016/j.crbiot.2021.12.004

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Ramp P, Lehnert A, Matamouros S, Wirtz A, Baumgart M, Bott M (2021) Metabolic engineering of Corynebacterium glutamicum for production of scyllo-inositol, a drug candidate against Alzheimer's disease. Metab Eng  67:173-185. https://doi.org/10.1016/j.ymben.2021.06.011

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Rolf J, Nerke P, Britner A, Krick S, Lütz S, Rosenthal K (2021) From Cell-Free Protein Synthesis to Whole-Cell Biotransformation: Screening and Identification of Novel α-Ketoglutarate-Dependent Dioxygenases for Preparative-Scale Synthesis of Hydroxy-L-Lysine. Catalysts 11:1038. https://doi.org/10.3390/catal11091038

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Rosenthal K, Becker M, Rolf J, Siedentop R, Hillen M, Nett M, Lütz S (2020) Catalytic promiscuity of cGAS: A facile enzymatic synthesis of 2’‐3’ linked cyclic dinucleotides. ChemBioChem 21: 3225-3228. https://doi.org/10.1002/cbic.202000433

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Schmitz J, Hertel O, Yermakov B, Noll T, Grünberger A (2021) Growth and eGFP-production of CHO-K1 suspension cells cultivated from single-cell to lab-scale. Front Bioeng Biotechnol 9: 716343. https://doi.org/10.3389/fbioe.2021.716343

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Schmitz LM, Hageneier F, Rosenthal K, Busche T, Brandt D, Kalinowski J, Lütz S (2021) Recombinant Expression and Characterization of Novel P450s from Actinosynnema mirum. Bioorganic & Medicinal Chemistry 42:116241. https://doi.org/10.1016/j.bmc.2021.116241

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Schmitz LM, Kinner A, Althoff K, Rosenthal K, Lütz S (2021) Investigation of vitamin D2 and vitamin D3 hydroxylation by Kutzneria albida. ChemBioChem 22: 2266-2274. https://doi.org/10.1002/cbic.202100027

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Schmitz LM, Schäper J, Rosenthal K, Lütz S (2019) Accessing the biocatalytic potential for C-H-activation by targeted genome mining and screening, ChemCatChem 11: 5766-5777. https://doi.org/10.1002/cctc.201901273

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Schneider P, Henßen B, Paschold B, Chapple BP, Schatton M, Seebeck F, Classen T, Pietruszka J (2021) Biocatalytic C3-Indole Methylation - A Useful Tool for the Natural Product Inspired Stereoselective Synthesis of Pyrroloindoles. Angew Chem Int Ed  https://doi.org/10.1002/anie.202107619

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Schultenkämper K, Fernandes de Brito L, Wendisch VF (2020) Impact of CRISPR interference on strain development in biotechnology. Biotechnol Appl Biochem 67: 7-21. https://doi.org/10.1002/bab.1901

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Seide S, Arnold L, Wetzels S, Bregu M, Gätgens J, Pohl M (2022) From Enzyme to Preparative Cascade Reactions with Immobilized Enzymes: Tuning Fe(II)/α-Ketoglutarate-Dependent Lysine Hydroxylases for Application in Biotransformations. Catal 12:354, https://doi.org/10.3390/catal12040354

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Sester A, Winand L, Pace S, Hiller W, Werz O, Nett M (2019) Myxochelin- and Pseudochelin-Derived Lipoxygenase Inhibitors from a Genetically Engineered Myxococcus xanthus Strain, J Nat Prod 82: 2544-2549. https://doi.org/10.1021/acs.jnatprod.9b00403

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Sester A, Stüer-Patowsky K, Hiller W, Kloss F, Lütz S, Nett M (2020) Biosynthetic plasticity enables production of fluorinated aurachins. ChemBioChem 21: 2268-2273; https://doi.org/10.1002/cbic.202000166

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Sgobba E, Wendisch VF (2020) Synthetic microbial consortia for small molecule production. Curr Opin Biotechnol 62: 72–79. https://doi.org/10.1016/j.copbio.2019.09.011

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Steenweg C, Seifert AI, Schembecker G, Wohlgemuth K (2021) Characterization of a Modular Continuous Vacuum Screw Filter for Small-Scale Solid–Liquid Separation of Suspensions. Org Process Res Dev 25, 926-940. https://doi.org/10.1021/acs.oprd.0c00550

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Volkenborn K, Kuschmierz L, Benz N, Lenz P, Knapp A, Jaeger KE (2020) The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis. Microb Cell Fact 19: 154. https://doi.org/10.1186/s12934-020-01404-2

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Wendisch VF (2020) Genome-reduced Corynebacterium glutamicum fit for biotechnological applications. In: Minimal Cells: Design, Construction, Biotechnological Applications. Lara AR, Gosset Lagarda G (Eds); Cham: Springer: 95-116. https://doi.org/10.1007/978-3-030-31897-0_4

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Wendisch VF (2020) Metabolic engineering advances and prospects for amino acid production. Metab Eng. 58: 17-34. https://doi.org/10.1016/j.ymben.2019.03.008

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Wendisch VF, Lee J-H (2020) Metabolic engineering in Corynebacterium glutamicum. In: Corynebacterium glutamicum – Biology and Biotechnology. Inui M, Toyoda K (Eds); Microbiology Monographs Vol 23. Springer, Cham: 287-322 https://doi.org/10.1007/978-3-030-39267-3_10

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Wendisch VF, Nampoothiri KM, Lee J-H (2022) Metabolic engineering for valorization of agri-and aqua-culture sidestreams for production of nitrogenous compounds by Corynebacterium glutamicum. Front. Microbiol. in press. https://doi.org/10.3389/fmicb.2022.835131

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Winand L, Schneider P, Kruth S, Greven N-J, Hiller W, Kaiser M, Pietruszka J, Nett M (2021) Mutasynthesis of physostigmines in Myxococcus xanthus. Org Lett 23, 6563-6567. https://doi.org/10.1021/acs.orglett.1c02374

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Winand L, Sester A, Nett M (2021) Bioengineering of anti-inflammatory natural products. Chem Med Chem 16, 767-776. https://doi.org/10.1002/cmdc.202000771

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Wierckx N, Miebach K, Ihling N, Hussnaetter KP, Büchs J, Schipper K. (2021) Perspectives for the application of Ustilaginaceae as biotech cell factories. Essays Biochem 65:365-379. https://doi.org/10.1042/EBC20200141

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Witte KE, Hertel O, Windmöller BA, Helweg LP, Höving AL, Knabbe C, Busche T, Greiner JFW, Kalinowski J, Noll T, Mertzlufft F, Beshay M, Pfitzenmaier J, Kaltschmidt B, Kaltschmidt C, Banz-Jansen C, Simon M (2021) Nanopore Sequencing Reveals Global Transcriptome Signatures of Mitochondrial and Ribosomal Gene Expressions in Various Human Cancer Stem-like Cell Populations. Cancers 13, 1136. https://doi.org/10.3390/cancers13051136

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Wolf S, Becker J, Tsuge Y, Kawaguchi H, Kondo A, Marienhagen J, Bott M, Wendisch VF, Wittmann C (2021) Advances in metabolic engineering of Corynebacterium glutamicum to produce high-value active ingredients for food, feed, human health, and well-being. Essays Biochem 65: 197-212. https://doi.org/10.1042/EBC20200134

 

Dr. Nadja Henke from CeBiTec, Bielefeld University, is featured as "Pioneer from NRW" in the first interview of a series organized by BIO.NRW. Dr. Henke initiated a spin-off from the laboratory of Prof. Volker Wendisch, coordinator of CKB, with the support of University of Bielefeld and by partners of CKB and CLIB. The BIO.NRW interview series is expected to feature other CKB members in the future.

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The CKB cross-campus collaboration on sustainable glutarate and ʟ-2-hydroxyglutarate production led to two publications with Carina Prell from the Wendisch lab at Bielefeld University as first author. For her work, Carina Prell was awarded a poster prize at the Metabolic Engineering 14 conference, July 11-15, 2021. In the collaboration, a sidestream of the Jäckering starch factory was studied as sustainable feedstock (Arthur Burghardt, Bielefeld), strains were developed and improved by adpative laboratory evolution (ALE) (Carina Prell, Bielefeld), mutations in the ALE strains were identified by genome re-sequencing (Tobias Busche, Christian Rückert, Bielefeld) and batch and fed-batch fermentations at the 2 L bioreactor-scale were perfomed (Florian Meyer, Bielefeld). A downstream process using extraction and reextraction was developed (Lea Nolte, Christoph Brandenbusch, Dortmund). Notably, among the candidate mutations identified by genome re-sequencing dedicated reverse genetics experiments established causality of the observed accelerated glutarate production, e.g., an amino acid exchange exchange in the large subunit of glutamine-2-oxoglutarate aminotransferase [1]. The concept could be transferred when the pathway was extended to ʟ-2-hydroxyglutarate production [2].

[1] Prell C, Busche T, Rückert C, Nolte L, Brandenbusch C, Wendisch VF (2021) Microb Cell Fact 20, 97. https://doi.org/10.1186/s12934-021-01586-3

[2] Prell C, Burgardt A, Meyer F, Wendisch VF (2021) Front Bioeng Biotechnol 8, 630476. https://doi.org/10.3389/fbioe.2020.630476

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On October 1st, 2020, nearly 70 scientists from all four partners met in Duesseldorf to discuss their results, but also their challenges and develop new ideas, solutions and shortcuts together. Input presentations from all three scientific work packages – resource efficiency, raw materials and health – highlighted the developments taken within the project up to now and a total of 21 posters invited all attendees to discuss one on one and in small groups about future possibilities to improve integration of process steps. After presenting and discussing the scientific status of the project a presentation on work package four – open innovation – was given. This work package is carried out by the cluster industrial biotechnology (CLIB) and aims at integrating stakeholders from industry and map out a strategy of continuation to sustainably use the project results. The meeting was summed up by a discussion on new ideas to further elaborate the project performance led by Prof. Volker F. Wendisch, project leader of the CKB.
The CKB receives funding for another one and a half years and the partners agreed on rescheduling this successful symposium twice to further improve integration and thus shorten the time from gene and enzyme to process and product in biotechnology.

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May 2020: The infrastructure CKB with its four competence centers (Bielefeld, Dortmund, Düsseldorf and Jülich) was presented by Volker F. Wendisch during an economic meeting of the regional branch of the Junior Chamber International. Christopher Henke presented the business perspective of industrial biotechnology during this tandem talk including the bicomer project.

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CKB expands and deepens network at the CIC2020, Feb 6-7, Düsseldorf
All four CKB competence centers (Bielefeld, Dortmund, Düsseldorf and Jülich) participated in the conference organized by CLIB and used the opportunity to meet members of CKB’s industrial advisory board, the IP board and the multinational and multisectoral network of CLIB. The project’s potential was even mentioned by Prof. Dr. Pinkwart, minister of economic affairs, digitalization and energy of NRW, who stressed the need for a faster market entry. He also mentioned the CKB as one of the projects tackling this challenge.

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Lively networking and efficient integration during the first CKB symposium, October 1, 2019, Düsseldorf

Innovations in biotechnology and bioeconomy still take a long time from university to the market, as multiple steps need optimisation. Traditionally, every step – from strain engineering to scale-up – is optimised one by another and often these individual process steps are not synchronised. The EFRE-funded project CLIB competence center biotechnology (CKB – CLIB Kompetenzzentrum Biotechnologie) aims at changing this way of developing innovation. Working groups from the universities Bielefeld, Dortmund, Duesseldorf and the Forschungszentrum Juelich are developing an integrated, virtual competence center to interlock process steps with each other and the whole innovation process with the local industry.
On October 1st, nearly 70 scientists from all four partners met in Duesseldorf to discuss their results, but also their challenges and develop new ideas, solutions and shortcuts together. Input presentations from all three scientific work packages – resource efficiency, raw materials and health – highlighted the developments taken within the project up to now and a total of 21 posters invited all attendees to discuss one on one and in small groups about future possibilities to improve integration of process steps. After presenting and discussing the scientific status of the project a presentation on work package four – open innovation – was given. This work package is carried out by the cluster industrial biotechnology (CLIB) and aims at integrating stakeholders from industry and map out a strategy of continuation to sustainably use the project results. The meeting was summed up by a discussion on new ideas to further elaborate the project performance led by Prof. Volker F. Wendisch, project leader of the CKB.
The CKB receives funding for another one and a half years and the partners agreed on rescheduling this successful symposium twice to further improve integration and thus shorten the time from gene and enzyme to process and product in biotechnology.

 

Prof. Dr. Volker Wendisch
Universität Bielefeld & CeBiTec
Universitätsstr. 25
33615 Bielefeld

volker.wendisch[at]uni-bielefeld.de